Plasmids, Mobile Elements and How Resistance Spreads · seed 1 · A4, ink-friendly. The answer key prints on its own page for grown-ups.
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Resistance travels as luggage

Science · Microbiology · ages 20-21
Name ______________________   Date ____________
  1. A plasmid copy passes from a living donor directly into a recipient during cell contact. Which process is this?

    • Uptake of naked DNA
    • Direct-contact conjugation
    • Inheritance during division
  2. Which description identifies a plasmid?

    • DNA that replicates separately from the chromosome
    • DNA that changes position within a chromosome
    • DNA that forms the bacterial chromosome and replicates with it
  3. A daughter cell can receive an unchanged copy of its parent's chromosome but fail to inherit a plasmid.

    Circle one:   True   False

  4. An intact plasmid confers resistance to drugs C and D. Drug C kills noncarriers, and the two genes stay linked. Why can resistance to D remain common even when D is absent?

    • Drug C favors carriers of the linked genes
    • Drug C induces D resistance in cells without the plasmid
    • Drug C favors the D gene after it separates from the plasmid
  5. DNA testing finds four resistance genes on one plasmid. All four function in the recipient. Why can one conjugation event give resistance to all four drugs?

    • Each transferred copy carries a different gene
    • The four genes transfer as separate copies of that plasmid
    • One plasmid copy carries the four genes together
  6. After drug withdrawal, plasmid carriers grow more slowly than noncarriers. Some daughters lose the plasmid, it gives no other benefit, and no transfer occurs. What trend is expected over many generations?

    • The fraction of plasmid carriers tends to increase
    • The fraction of plasmid carriers tends to stay unchanged
    • The fraction of plasmid carriers tends to decrease
  7. Resistance genes are found only on plasmids, never on bacterial chromosomes.

    Circle one:   True   False

  8. A resistance-carrying transposon moves from a chromosome to a plasmid inside the same bacterium. No DNA enters or leaves the cell. What does this demonstrate?

    • Conjugation between two cells
    • Transposition within one cell
    • Transduction between cells
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Answer key

For grown-ups. Fold this page away before handing over the rest.

Resistance travels as luggage W1-mt_Vk0qOY64qv-s1

  1. Direct-contact conjugation · Direct donor-recipient DNA transfer is conjugation. The observation identifies the route, not a universal ranking of spread speeds.
  2. DNA that replicates separately from the chromosome · A plasmid is DNA that can replicate separately from the chromosome. It still uses machinery in the host cell.
  3. True · Chromosome and plasmid inheritance can differ. Plasmid loss does not by itself require a chromosome change.
  4. Drug C favors carriers of the linked genes · Drug C favors plasmid carriers. The D-resistance gene stays linked on the same intact plasmid, so it can be retained too.
  5. One plasmid copy carries the four genes together · The four genes are already linked on one plasmid. A copy can deliver them together, and the prompt states that they function in the recipient.
  6. The fraction of plasmid carriers tends to decrease · The stated conditions favor noncarriers: they grow faster, loss creates more of them, and transfer does not replenish carriers.
  7. False · Resistance genes can occur on plasmids or chromosomes. A resistance trait alone does not establish the gene's location.
  8. Transposition within one cell · The segment changes DNA location within one cell. This demonstrates transposition, not transfer between cells.
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