How does fluorescent antibody staining work?

• Cells are fixed, then antibodies carry dyes to the target
• Genes are edited so proteins glow by themselves
• Cells are frozen and viewed with electrons only

You must watch one protein move in a living cell over an hour. Which tool fits?

• An antibody stain on fixed cells
• A bright field photo with no label
• A genetic tag fused to its gene

If two colours overlap in an image, the two proteins must bind to each other.

Which is one way a tag can change its protein behaviour?

• It always doubles the protein count
• It removes the cell nucleus
• It can block a binding site or slow movement

What does a two-colour overlap claim, and what does it leave open?

• Direct binding proven forever
• Shared place and time; whether they touch stays open
• Identical amounts of both proteins

Bleed through may be faking your overlap. Which control rules it out?

• A brighter merged image
• A higher zoom with no controls
• Single colour controls imaged alone

You need a movie of traffic over time, but someone suggests fixed antibody snapshots. Why refuse?

• Snapshots are dead cells; only tags give live timing
• Antibodies never bind specifically
• Movies are always less sharp than snapshots

The tagged protein crawls slower than untagged protein in a control. What do you conclude?

• The microscope is broken and slow
• Slower always means more accurate tracking
• The tag disturbs movement; trust the untagged behaviour