Straight lines that name the blocker

Science · Biochemistry & Molecular Biology · ages 18-19

Name ______________________ Date ____________

What does the y intercept equal?
- Vmax itself
- Km divided by two
- One over Vmax
The double reciprocal plot graphs which two quantities?
- Rate against substrate
- One over rate against one over substrate
- Log rate against time
Adding substrate can overcome competitive inhibition.
Circle one: True False
Lines meet on the x axis with Vmax fallen and Km steady. Which type?
- Competitive
- Non competitive
- Uncompetitive
Lines meet on the y axis with Vmax steady and Km risen. Which type?
- Non competitive
- Uncompetitive
- Competitive
Lines run parallel with both constants lower. Which type?
- Competitive
- Non competitive
- Uncompetitive
Why can extra substrate not rescue non competitive inhibition?
- The active site shrinks
- Substrate turns into blocker
- The blocker binds a separate spot
A transform bends instead of straightening. What should you suspect?
- A cooperative S shaped enzyme
- A broken ruler
- Too much substrate

Answer key

For grown-ups. Fold this page away before handing over the rest.

One over Vmax · The y intercept reads one over Vmax directly.
One over rate against one over substrate · Both axes carry reciprocals, which straighten the hyperbola.
True · Flooding the active site washes the blocker out.
Non competitive · A shared x intercept with lower Vmax is non competitive.
Competitive · A shared y intercept is the competitive fingerprint.
Uncompetitive · Parallel lines with both Vmax and Km down mark uncompetitive binding.
The blocker binds a separate spot · Flooding the active site cannot dislodge a blocker bound elsewhere.
A cooperative S shaped enzyme · Cooperative enzymes draw S curves that the transform cannot flatten.