Where is Km on the rate curve?

• The peak of the curve
• The point where rate hits zero
• The substrate level giving half the maximum

What does the steady state assume about the complex?

• It never forms at all
• It forms as fast as it reacts
• It grows without limit

Raising substrate past saturation keeps raising the rate.

Circle one:   True   False

What sets Vmax?

• Turnover per site times total enzyme
• Substrate left at the end
• The speed of product leaving only

Km equals which mix of rate constants?

• Binding over breakdown
• Breakdown plus chemistry over binding
• Chemistry alone

When does the steady state hold?

• Late, when substrate runs out
• Early, while substrate far exceeds enzyme
• Only with no enzyme present

Why can extra substrate never pass Vmax?

• Km blocks the way
• Product pushes back harder
• Sites are all busy already

A mutant raises the chemistry rate but keeps binding fixed. What happens to Km?

• It rises
• It falls to zero
• It stays exactly fixed