Which order do flask phases run in?
- Log, lag, death, stationary
- Lag, log, stationary, death
- Stationary, death, lag, log
What is generation time?
- Doublings per hour
- The time per doubling
- The lag before growth
Binary fission splits one cell into two matching daughters.
Circle one: True False
Why does the log phase look straight only on a log axis?
- Each generation multiplies by a fixed factor
- Cells pause on plain paper
- The ink spreads evenly
A culture rises from 100 to 800 in 6 hours. Type the generation time in hours.
Answer: ______________
What does a chemostat control that a closed flask cannot?
- Faster mutations on demand
- Growth without any medium
- Steady growth by matched feeding and outflow
Strain A doubles every 30 minutes and strain B every 60 minutes. Which grows faster and why?
- B, since longer time means more cells
- A, since it doubles twice as often
- Both tie after one hour
A culture doubles every 40 minutes. Starting from 25 cells, how many are there after 2 hours?
Answer: ______________
Counting cells that double W1-mt_ltzgjIBytk-s1
- Lag, log, stationary, death · Lag, then log doubling, then stationary, then death.
- The time per doubling · Generation time is the time one doubling takes.
- True · Each doubling makes two identical cells from one.
- Each generation multiplies by a fixed factor · Constant factor growth plots curved on plain axes and straight on log axes.
- 2 · That is three doublings, and 6 hours divided by 3 is 2.
- Steady growth by matched feeding and outflow · Matched inflow and outflow hold cells in one steady phase.
- A, since it doubles twice as often · Shorter generation time means more doublings per time.
- 200 · Two hours holds three doublings: 25 to 50 to 100 to 200.