Every strong analogue carries an added side chain. The weak ones lack it. What do you conclude?

• It weakens every compound
• It has no role at all
• The target requires that side chain

To learn what one substituent does, which set do you make?

• Analogues that differ at that spot alone
• Analogues that change every spot at once
• Copies of the hit with no changes

A potency gain that disappears under new assay conditions was never real binding.

Circle one:   True   False

Analogue A is bigger and slightly stronger. Analogue B is smaller with better efficiency. Which is the better lead?

• Analogue B, by compact strength
• Analogue A, by raw potency
• Neither can be judged at all

A compound looks potent but forms clumps in solution. What do you suspect?

• Strong binding to the target
• An aggregation artefact
• A cleaner assay signal

An analogue changes two spots at once and gains potency. What can you claim?

• Each spot helped equally
• Nothing about either spot alone
• The worse spot carried the gain

Each added atom raises potency, but efficiency keeps falling. What should the team do?

• Keep adding atoms for more potency
• Stop adding bulk and seek compact gains
• Restart screening from zero

All strong analogues share a side chain, but one weak analogue has it too. What do you conclude?

• The side chain is useless
• The table must be thrown out
• It helps but is not the whole story